Fluorescence Brightness calculator

Intrinsic brightness B = ε · Φ for fluorophore comparison.

Category: Imaging

Formula: B = ε · Φ

Inputs

InputParameterUnitDefault
ε (M⁻¹cm⁻¹)eps73000
Quantum yield Φphi0.79

Result with the default inputs

  • Brightness: 57670 M⁻¹cm⁻¹

Open it with your own values: put each input in the address as a parameter, for example /calculators/fluorescence-brightness?eps=73000. A unit other than the listed one goes in <parameter>_unit.

Read more: Wikipedia: Fluorophore

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