---
title: "Fluorescence Brightness calculator — AperiOptic"
description: "Intrinsic brightness B = ε · Φ for fluorophore comparison. Free online optics calculator, with the formula and code snippets."
url: "https://aperioptic.com/calculators/fluorescence-brightness"
---

# Fluorescence Brightness calculator

Intrinsic brightness B = ε · Φ for fluorophore comparison.

Category: [Imaging](https://aperioptic.com/calculators)

Formula: `B = ε · Φ`

## Inputs

| Input | Parameter | Unit | Default |
| --- | --- | --- | --- |
| ε (M⁻¹cm⁻¹) | `eps` |  | 73000 |
| Quantum yield Φ | `phi` |  | 0.79 |

## Result with the default inputs

- Brightness: 57670 M⁻¹cm⁻¹

Open it with your own values: put each input in the address as a parameter, for example [`/calculators/fluorescence-brightness?eps=73000`](https://aperioptic.com/calculators/fluorescence-brightness?eps=73000). A unit other than the listed one goes in `<parameter>_unit`.

Read more: [Wikipedia: Fluorophore](https://en.wikipedia.org/wiki/Fluorophore)

[All optics calculators](https://aperioptic.com/calculators)

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The interactive page: https://aperioptic.com/calculators/fluorescence-brightness (AperiOptic runs in the browser; free, no account). Every page of the site as Markdown: https://aperioptic.com/llms.txt