Multiphoton PSF Volume calculator
Lateral & axial PSF for two-photon excitation.
Category: Laser & Ultrafast
Formula: wxy = 0.320·λ/(√2·NA) (NA ≤ 0.7), 0.325·λ/(√2·NA^0.91) (NA > 0.7) · wz = 0.532·λ/(√2·(n-√(n²-NA²)))
Inputs
| Input | Parameter | Unit | Default |
|---|---|---|---|
| λ | lam | nm | 920 |
| NA | NA | 1 | |
| Index n | n | 1.33 |
Result with the default inputs
- Lateral wxy: 211.4249 nm
- Axial wz: 763.7674 nm
Open it with your own values: put each input in the address as a parameter, for example /calculators/multiphoton-psf-volume?lam=920. A unit other than the listed one goes in <parameter>_unit.
Read more: Wikipedia: Two-photon excitation microscopy